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The qPCR assays were developed utilizing unique sequences of the R. typhi and R. felis outer membrane protein B genes (ompB) to design the specific primers and molecular beacon probes.
The unique sequences of the four batches were used for aligning to known miRNA sequences from miRBase v15; between 3,300 and 5,000 sequences were obtained from various samples (Table 1).
Comparisons of amino acid variation between the full-length unique sequences of the 10 WNV proteins showed that C had the highest range of amino acid differences across the sequences (up to 23%), while NS4b had the lowest (up to 8%) (Table 1).
There were 12 unique sequences of the nuclear histone (h3) gene, 16 unique sequences of the nuclear 18 S gene, and 18 unique sequences of the nuclear 28 S gene.
We constructed here a first reference genome comprising an estimated two thirds of the unique sequences of the I. ricinus tick, the most important European disease vector.
We identified over 900 representatives (659 unique sequences) of the motif in the marine metagenome in addition to the instance in ' Cand.
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The methodology makes use of the unique sequence of the G11 gene upstream of C4A and the sequence of a 6.4 kb retrotransposon, the human endogenous retrovirus HERV-K(C4), which is present in intron 9 of C4A but absent in the case of the deletion.
As reported previously the unique sequence of the Xist gene is not conserved and evolves very rapidly [2], [15].
From this data, the QPIHPNNM peptide was the most unique sequence of the three longer sequences recovered and was synthesized for further testing.
The unique sequence of the transgene can be used to easily locate the insertion site.
Picky prioritized the unique sequence of the identified oligonucleotide probes while maintaining a uniform probe-target melting temperature.
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