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Four small interfering RNAs (siRNAs) were designed against unique sequences in the K6a 3′-untranslated 3′-untranslated
Previous works only give the mean of the number of unique sequences in the library, and they can only handle equal molar ratio of the four nucleotides at a small number of mutation sites.
Moreover, there are currently about nineteen thousand unique sequences in the NCBI UniGene database for P. taeda.
It might not sacrifice assembly accuracy if some of the unique sequences in the Newbler assembly were joined into contigs by lowering the assembly stringency.
The simulation was run over a range of cluster densities and with a varying numbers of unique sequences in the cluster calling bases to assess the impact of reduced sequence diversity on the efficiency of cluster calling.
Tissue-specific alternative splicing may have resulted in different RNA transcripts from the same DNA gene [44], and is a plausible reason for the high abundance of unique sequences in the transcriptomic assembly.
After removal of rRNA, tRNA, snRNA, snoRNA and repeat sequences characterized in Rfam [16] and Genbank [17], a total of 3.8 5.3 million unique sequences in the range of 18 25 nt remained in the four batches and these were used for further analyses (Table 1).
We obtained 194 unique sequences in the F library and 115 unique sequences in the R library.
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It thus appears that the entire ovalbumin mRNA is primarily transcribed from a unique sequence in the chick genome.
The only site of unique sequence in the KIR locus is in the 14kb intergenic region that separates 3DP1 from 2DL4 and divides the locus into centromeric and telomeric parts of similar size [45].
When grouping duplicate sequences, it is important to propagate annotations, and keep track of how much support there is for each unique sequence in the underlying data.
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