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Details of the three methods are as follows: Method 1, miRDeep program was used to identify porcine miRNAs from large pool of unique sequenced transcripts from our Solexa deep sequencing.
The database hosts >29 472 unique single nucleotide variants from 469 unique sequenced strains of M. tuberculosis (Supplementary Data S4).
We used 238,538 sRNA sequences (of which 98,035 were unique) sequenced by the 454 technology (Table 1) [ 19, 20].
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Parasites are living organisms that feed off of other organisms; viruses are unique sequences of genomic material which can cause a disruption or aberration of normal biological function.
So for an 8,000-character 8,000-characterre looking at at leasequence0 per unique sequence.
However, some atypical centromeres spontaneously form on unique sequences.
These clones have unique sequences after sequencing their VHH fragment.
The bivalent library yielded numerous unique sequences, while the monovalent library yielded fewer unique clones.
Each EST library was independently assembled and 1300 3000 unique sequences were identified in each species.
A unique sequence was chosen for each pathogen and used for primer design.
For 86,732 unique sequences among them, a total of 331,368 primer pairs were designed.
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