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The methodology makes use of the unique sequence of the G11 gene upstream of C4A and the sequence of a 6.4 kb retrotransposon, the human endogenous retrovirus HERV-K(C4), which is present in intron 9 of C4A but absent in the case of the deletion.
As reported previously the unique sequence of the Xist gene is not conserved and evolves very rapidly [2], [15].
From this data, the QPIHPNNM peptide was the most unique sequence of the three longer sequences recovered and was synthesized for further testing.
The unique sequence of the transgene can be used to easily locate the insertion site.
Picky prioritized the unique sequence of the identified oligonucleotide probes while maintaining a uniform probe-target melting temperature.
For each strain, every unique sequence of the ptxP, fim3 and prn loci received a distinct allele number.
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The unique sequence of these three genes suggests they may be unique to legumes.
The unique sequence of this site does not resemble known SHP1 interaction motifs of cellular proteins.
The qPCR assays were developed utilizing unique sequences of the R. typhi and R. felis outer membrane protein B genes (ompB) to design the specific primers and molecular beacon probes.
The combined unique sequences of the two cDNA clones yielded 1395 base pairs and contained the entire coding region for calmodulin, 94 base pairs of the 5'-nontranslated region, and the entire 3'-nontranslated region of 857 base pairs.
The unique sequences of the four batches were used for aligning to known miRNA sequences from miRBase v15; between 3,300 and 5,000 sequences were obtained from various samples (Table 1).
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