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Telomerase is a unique reverse transcriptase ribonucleoprotein complex that maintains a steady state of telomere length by synthesizing TTAGGG repeats at the ends of chromosomes.
The first round of the nested RT-PCR was performed in three separate reactions using a common forward primer located in exon 1 (F1andand a unique reverse primer located in exons 3 (R13), 5 (R15) or 8 (respectivelyTabley (Table S1).
Each sample was assigned a unique reverse primer containing the index used for barcoding.
These 4 forward primers were individually coupled with the unique reverse primer (see above).
Telomerase is a unique reverse transcriptase, expressed almost exclusively in >90% of cancer cells.
Four unique forward barcodes (F-barcode) and eight unique reverse barcodes (R-barcode) were used to code the 32 samples.
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Some annotation-based metrics were fully consistent when computed over both contigs and unigenes, including the number of unique reverse-annotations and the number of reciprocal best hits.
Scapular notching is a complication unique to reverse total shoulder arthroplasty (rTSA), although its clinical implications are unclear and remains controversial.
Type 2 diabetes is a disease affecting 415 million people globally and Virta, a Silicon Valley-based health startup, believes it has something unique to reverse it – remote monitoring of everything you eat.
Try this: They doctor the local water to match the mineral content of other famous brewing cities. "We use a unique RO (reverse osmosis) system that allows us to rebuild the water we use in our beer to any profile we'd like," Byron said.
Site-directed mutagenesis was done using pUC8 containing the whole BSCTV genome inserted at the EcoRI unique site (reverse) with the Quick Change Site-Directed Mutagenesis Kit (Strategene, CA, USA).
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