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Seven functional modules are divided by strategically placed unique restriction sites.
Twitter, Facebook and other social media have been used to get women drivers on the roads on Saturday in a marathon push against this unique restriction.
To test this system, a His tag with an additional unique restriction site (for selection and assay digests) was recombined into the pCONGA HSBD site to create pCONGAH.
A synthetic gene for the human motilin receptor containing 33 unique restriction sites was designed and stably coexpressed in HEK293 cells with the bioluminescent Ca2+ indicator protein aequorin.
Two unique restriction sites and a copy of the transcription regulatory sequence for ORF6 (TRS6) were inserted between ORFs 1b and 2a, yielding a general purpose expression vector.
Standard DNA ligations are performed at seven unique restriction sites 5′ to the lacZ gene on high-copy ColE1 plasmids suitable for double- or single-strand DNA sequencing.
Unique restriction sites 5′ and 3′ of the selection marker have been reserved for insertion and simultaneous transformation with two transgenes.
Our resulting modified genomes have several times more unique restriction sites and reduce the maximum gap between adjacent sites by three to nine-fold.
The normal E1A regulatory elements have been replaced by a convenient set of unique restriction enzyme sites, allowing for introduction of gene regulatory cassettes with relative ease.
Sequences of the unique restriction modification systems (RM) can serve as a kind of molecular 'barcode', facilitating specific strain identification in the environment.
I will only see the work as documentation, putting a unique restriction on my process and capabilities.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com