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DNA sequence analysis of 384 clones indicated the presence of unique inserts and a random distribution of sequences when aligned to the human genome sequence.
The Mariner and Tn7 transposon libraries contained 7381 and 2169 unique inserts, respectively.
In addition, both archaeal actins and Arp3 possess unique inserts that are missing in eukaryotic actins.
The CRISPR are interspersed in the sense that they contain short unique inserts (spacers) embedded within each palindromic repeat unit.
The archaeal actin-like proteins have, approximately, the same overall lengths as Arp3, with some shared and some unique inserts.
UAS- dPSA lines were created using pUAST transgenesis (PSA3- 4, PSA3- 7 and PSA8- 10 were unique inserts, each on the third chromosome).
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The unique insert domain of myosin VI neck is one such example where the insertion has resulted in reverting the direction of motion of the protein.
Such improvement was achieved by using modified BCH-based data hiding and unique inserting-removing strategy.
Our results (submitted) plus Shi's observations (Shi et al., 2015) revealed an unexpected contribution of this unique inserting sequence in constituting an extra-ligand binding motif for FadR regulatory protein.
Unique insert sequences were identified within each genetic selection with few clones containing the same insert sequence.
In summary, the cloning strategy described here, that we call 'Golden Gate' cloning, combines the convenience and efficiency of currently used recombination-based cloning systems with unique insert precision.
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