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The RPKM method was used to estimate the unique gene expression levels [ 63].
We used the reads per kb per million reads (RPKM) method to calculate unique gene expression levels [ 51].
A heat map for these genes indicated the unique gene expression levels in yHCC, with 225 probe sets being predominantly up in yHCCs (Table 2) while another 84 being down.
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We saw strong liver-specific effects of single nucleotide polymorphisms on both methylation levels (28,447 unique CpG sites (meQTL)) and gene expression levels (526 unique genes (eQTL)), at a false discovery rate (FDR) < 0.05.
In the following paper, a unique design to quantify gene expression levels at the nanoscale in a continuous flow system is presented.
Such epi-allelic hypomorphic analysis using different RNAi triggering sequences empirically determined to knockdown gene expression to distinct levels, provides a unique opportunity to correlate gene expression levels with phenotypic outcome [ 3].
They found that each tissue displays a unique combination of detectable Hox gene expression levels which is altered when normal cells changes to malignant cancer cells [ 26- 28].
By analysing transcription levels of An. coluzzi from VK7 in both 2011 and 2012, this provided a unique opportunity to determine whether gene expression levels were simultaneously rising with pyrethroid resistance in a single wild-caught population of An. coluzzi from south-west Burkina Faso.
Given the correlations between genotypes and methylation levels, we also estimated the unique contributions of the two on gene expression levels (Additional file 12).
This was also the case in Jonsson et al, where each patient had a unique profile of clinical annotations and immunological gene expression levels (Additional file 7).
Through the consortial efforts of participants in the HESI Committee on Genomics, a unique resource has been assembled that connects gene expression levels in the liver and kidney of rats from the control arms of numerous, diverse toxicogenomics studies to specific study meta-data.
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