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Third, the first backtracking step appears to be unique from further backtracking steps in two aspects.
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To reduce the times that we identified a sequencing error as a transcript from a poorly expressed gene, we required that any sequence difference be seen at least twice in the entire soybean EST collection, thus removing both sequences with random errors and truly unique transcripts from further analysis.
Reads that mapped to non-coding RNA sequences and reads that did not map to unique positions were excluded from further analysis.
To make the libraries meaningful, reads that appeared less than four times per million unique mapped reads were eliminated from further statistical analysis.
These unique tags and their corresponding sequence reads were also excluded from further analysis.
For identification of novel miRNAs, unique sRNAs with more than 10 genomic hits were removed from further analysis.
Each CYP2D6 allele whose unique variants are not supported after previous filtering steps are removed from further consideration.
The final library resulted from further curation and scrutiny of those peaks and ultimately consisted of 327 unique compounds.
Further analysis of BESs provided 16,086 unique (non-repeat) BES, while 37,230 BESs were found to contain repeat BESs and were therefore excluded from further mapping (Fig. 6).
Of these 60 unique compounds, the parental cell imaging assay identified 23 independent samples as potentially fluorescent, and these were excluded from further followup.
couesi from further south.
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