He developed a unique lexicon and catalog of insults for the character.
It can be interesting to catalog unique adaptations (such as the colour bands on coral snakes or the human ability to throw objects over long distances), but evolutionary theorists have a higher goal to uncover common principles underlying those diverse adaptations.
As a simple model for analyzing adaptation, experimental evolution of microorganisms provides the unique opportunity to catalog, monitor the dynamics, and measure the reproducibility of adaptation in real time.
Some items previously thought to be "probably unique," according to the catalog description, now turn out to have long-lost twins, including a block of four three-cent stamps from 1867 with a marginal imprint reading "National Bank Note Co.
It has been suggested that 3'cDNA 454-sequencing can enable resolution of a catalog of unique transcripts, eliminating overestimation associated with shotgun sequencing of multiple non-overlapping 454-ESTs per transcript [ 20, 25].
Here, we show that 454 sequencing of 3' cDNA fragments from a normalized library constructed from pooled RNAs provides a powerful method to rapidly build up an extensive catalog of unique transcripts that can be successfully used to develop a microarray for large scale analysis of gene expression in any species, without the need for prior sequence knowledge.
To assess the use of 454-derived unigenes to develop an extensive catalog of unique transcripts without the need for prior sequence knowledge, the two sets of unigenes were mapped to the assembled grape genome sequence [ 18] using the BLAT algorithm, which takes into account introns by allowing the splitting of unigenes (Table 3).
The method we developed is based on 454 sequencing of 3' cDNA fragments from a normalized library constructed from pooled RNAs to generate, through de novo reads assembly, a large catalog of unique transcripts in organisms for which a comprehensive collection of transcripts or the complete genome sequence, is not available.
The other set was originally built in Blekhman et al. [ 10] and updated in Primate Orthologous Exon Database (POED), which includes a catalog of unique, non-overlapping, 1 1 1 orthologous exons of human, chimpanzee and rhesus macaque indentified through local alignment from Ensembl annotation.
