The F is for fulfillment (are employees happy about their assignments?), U is for uniqueness (are employee's unique talent aligned with their assignments?) and N is for next (are employees excited for what's next?).
The rescue method calculates similarly the number of unique reads aligned to a contig, but the multi-mapped reads were counted fractionally proportional to the number of different map sites, the expression level and the length of the contig.
We reviewed the additional "fill in" alignments and found only a possible 2 kb of unique additional aligned sequence that could have been used for SNV identification.
The ratios of unique reads aligned with zero, one, and two mismatches were 36 %, 32, and 32%, respectively.
Characterisation of this library indicated that most inserts were unique and aligned to different regions of the human genome.
GRMZM2G037875_T03 is the CDS with greatest number of unique siRNAs aligned with it.
Quantifying expression levels: expression levels from unique reads aligned by Bowtie were calculated as RPKM values (Mortazavi et al., 2008).
There were very limited unique SNPs aligned at Interval 20 27 of all the three cultivars, suggesting the region may contain the centromere, usually characterized by fewer genes.
For each window, the number of intervals with aligned reads, the total number of reads aligned, the number of unique reads aligned, the fraction of intervals on the Watson strand, and the higher number of aligned reads at a single interval within the window are reported.
In the wild-type library 26% of the total reads (16% of the unique reads) aligned to this retroposon with approximately twice as many reads originating from the antisense strand.
Similarly, approximately 14% of unique reads aligned to X chromosome were mapped to the 60 kb region spanning the gap (30 kb on each side; a total of 1.8% of the pseudomolecule).
