The final layout contained 18,867 unique bean sequence probes and 11,205 soybean UniGene probes, along with positive and negative controls, for a total of 30,150 different features available on the microarray.
Unique "micro-bean sprouts" with "nano-tails" were electrohydrodynamically prepared from poly[(propylmethacryl-heptaisobutyl-polyhedral oligomeric silsesquioxane -co- methylmethacrylate)] (POsilsesquioxane -co- methylmethacrylate
One limitation to consider is that distinct P. vulgaris genes may exist without a soybean homologue to utilize during hybridization and thus a cross-species microarray may exclude unique dry bean transcripts.
We used BLAST results of bean unique sequences against Arabidopsis proteins, to identify bean sequences homologous to Arabidopsis transcription factor genes from PlnTFDB http://plntfdb.bio.uni-potsdam.de/v3.0/[ 35].
When compared to Medicago, soybean and lotus, 28% (16,525) of the unigenes are unique to common bean whereas only 0.43% (254) of the unigenes are unique to common bean when compared to Arabidopsis and rice.
For faba bean, a total of 549 ESTs (222 unique matches against faba bean unigene set) and 3,684 nucleotides (1,277 unique matches against faba bean unigene set) were found be common between Genbank and transcriptome data generated from the current study (Additional file 13, sheets 3-4).
Each bar is a careful experiment of roasting, conching and aging, with different beans exhibiting unique perfumes and flavour notes.
The protein expression patterns in P. pastoris (GS115) remained unchanged when growth was conducted in a medium containing extruded bean as unique nitrogen source.
While extra-dark roasts have become "sophisticated," many coffee lovers prefer a medium or medium dark roast that doesn't scorch away the unique flavors of the bean.[5].
A previous study involving common bean EST analysis also suggested a unique gene expression pattern in common bean nodules compared to nodules of other legume species such as Medicago truncatula and soybean [ 6].
