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As a result, it is not always possible to obtain a single unique alignment for a given read.
PF Reads were de-multiplexed using the Barcode Splitter in FASTX-toolkit and the reads from each library were mapped to mouse genome build mm10 with RefSeq gene annotation, using Tophat 2 software, allowing only unique alignment for each read.
Our notion of mappability also includes a "safety margin", so that a mappable region guarantees not only a unique alignment for the reads matching the sequence, but also that no read with one or two base call errors or SNPs relative to the reference genome could be uniquely mismapped to this region.
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Only unique alignments for each read were reported (-n 1 -Q); otherwise, default parameters were used.
Andrew Simms of the New Economics Foundation said: "We face a unique alignment of economic and environmental interests.
For instance, features such as the presence of repetitive DNA make the unique alignment of sequence reads difficult or impossible.
Using this database, we successfully identified unique alignment positions for 51 of 436 sequenced RAD loci (11.0%).
The Pt-ACNT arrays exhibit unique alignment, which allows for rapid gas diffusion and chemisorption on the catalyst surfaces.
Identification of M regions is performed after discarding the unique alignments from total alignments.
The conversion of the genome from a 4-base alphabet to a 3-base alphabet lengthens the number of bases required for unique alignment.
Sequence lengths generally range from 25 50 bp (short sequences), which is sufficient for unique alignment to a reference genome [ 6].
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CEO of Professional Science Editing for Scientists @ prosciediting.com