Sentence examples for unique alignment from inspiring English sources
Each aligned read was assigned a mapping quality that indicates whether the read has a unique alignment or can be aligned to multiple positions in the genome [ 22].
On average, 36 million uniquely aligned reads were obtained per data set, representing an average unique alignment rate of 76%.
Reads were trimmed with Trim Galore v.0.1.4 to a Phred quality > 20 and minimum length of both pairs of 25 bp, and the quality trimmed reads were aligned to the swine reference genome using the unique alignment option of Mosaik Aligner v.1.1.0017 [ 40].
We then aligned the Illumina reads to the D. melanogaster exome, requiring unique alignment to a single location and allowing one, two, or three mismatches.
Andrew Simms of the New Economics Foundation said: "We face a unique alignment of economic and environmental interests.
This unique alignment, Gates and McConnell believed, would offer the best chance that the intelligence community would ever have to reform itself.
For gene expression analysis, we only examined transcripts with a reciprocal hit in the other genotype where >75% of the aligned reads were unique alignments, not aligning to any other transcripts.
We show ∼1.5% gain in unique alignments shown if mismatch tolerant aligner settings are used, although a more substantial difference could be seen when perfect matches were required.
Within each sample, an average of 5.5 M of these reads were unique alignments, while an average of 13.9 M mapped reads also aligned to other transcripts.
To determine the most likely genomic source of sequences aligning antisense within the 3' UTR clusters, these sequences were extracted and re-aligned to the genome under strict conditions (up to one mismatch, unique alignments only).
These sequences were quality-filtered and mapped to the Trichoderma reesei 2.0 reference genome, available from the JGI Genome Portal (http://genome.jgi-psf.org/Trire2/Trire2.home.html), using the Bowtie aligner version 0.12.8 [ 54], allowing for two mismatches and only unique alignments.
