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Using "union" mode a fusion image was generated, including the visible spots for each 2-D gel.
Predictions using the UNION mode of miRGen add additional proteins, but the large overlap of predicted targets between the three microRNAs remains.
A fusion gel image was subsequently generated using union mode, which is a weighted arithmetic mean across the entire gel series.
MiRGen was used in the UNION mode (collection of all predictions from 6 algorithms) as well as in the INTERSECTION mode (presenting all target genes predicted by algorithms PicTar AND TargetScan), the latter resulting in less but more reliable predictions.
Subsequently, a fusion image was generated employing "union" mode, creating a protein pattern containing all spots from all 2D gels (cortex as well as hippocampus at all age stages except ED16, internal standard gels were not included) within the project.
We used HTSeq to quantify the number of reads aligned to each gene using the "union" mode.
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Counts were generated using htseq-count v.0.5.3p9 with the default "union" overlapping mode.
Numbers of reads in genes were counted by the HTSeq-count tool using corresponding gene annotations, and the "union" resolution mode was used [ 32].
After aligning all image sets in position by using the internal standard (Cy2), all samples of a set were merged in order to create a fused image (2D proteome map) in union fusion mode [ 39].
The wage-labor relation can be influenced by income policies related to trade unions and modes of employment.
Privatization became the policy of governments.16 Privatization transactions increased, and this also played a role in decreasing the power of trade unions.17 The mode of economy in the developed countries became excessively open and oriented to international competition.
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