From each sample, 15,000 viable MAC-T cells were analyzed after setting a live cell gate based on the forward and side scatter profiles of uninfected MAC-T cells.
Investigating femoral marrow and thigh muscles as seemingly uninfected tissue, we found only a small side difference in the muscles and of a size (about 11%) considerably smaller than the difference between infected and non-infected tissues.
In uninfected cells, a tuft of APP staining to one side of the nucleus overlapped with TGN46, and thus likely represents the trans-Golgi network (Figure 2A) [36], [36].
Interestingly, while apical infection of T84 cells failed to trigger significant increases in IL-8 release above basal levels in uninfected cells, introduction of the bacteria to the basolateral side induced a ∼30 50-fold ∼30 50-foldincrease
As transgene expression was restricted to the injected side in all animals, we used the uninfected contralateral hemisphere as well as EGFP transduced sections to set the lower threshold value to correct for non-specific background.
The slight increase in the size of the lymphocytes (indicated by an arrow in Fig. 1 forward scatter and side scatter plots) in infected frogs compared to the uninfected controls suggests activation and consequent blasting of lymphocytes in the spleen as observed in mammals and ectothermic vertebrates (21).
For example, the left-hand side of (1) defines the rate of change of the uninfected CD4+ T cells.
At 1 and 4 hours post-release, a normal distribution of α-tubulin staining was noted in uninfected Vero cells; specifically, microtubules radiated from the microtubule-organizing center on one side of the nucleus (Fig. 8A).
Infected and uninfected erythrocytes were gated on the basis of their forward scatter (FSC) and side scatter (SSC) signals.
