Exact(1)
Databases for the various comparisons were comprised of publicly available and assembled sequences from wheat where care was taken to include only sequences derived from "clean", uninfected material, and from various fungi and oomycetes such as the COGEME db, http://cogeme.ex.ac.uk/[ 54].
Similar(57)
For the first time, such an approach, with an internal analytical control, is described in a diagnostic procedure for plant pathogenic phytoplasmas enabling distinction between uninfected plant material and false-negative results caused by PCR inhibition.
We confirmed the position of these eukaryotic-like secA genes in the Wolbachia genome, and their absence from uninfected host material, using PCRs spanning wBol1_1089-wBol1_1091 and wBol1_1092-wBol1_1093, on infected and antibiotic-treated lines of H. bolina.
Experimental controls for ic and ip transmissions were inoculated under the same experimental conditions using identical quantities of uninfected mouse brain material.
Twenty-four Large White×Landrace pigs were inoculated with 105 PFU of FMDV C-S8c1 and four pigs were used as uninfected controls (see Materials and Methods).
Mice were inoculated intradermally in the ear with either 5000 P. yoelii sporozoites, an equivalent amount of salivary gland material from uninfected mosquitoes, the bites of 10 P. yoelii-infected mosquitoes, the bites of 10 uninfected mosquitoes or medium alone.
In bioassays of TSE infectivity, the possibility that trace levels of infectivity in tissues may represent postmortem or laboratory contamination of uninfected tissues with infected material has to be considered.
At day 21 PI (4 weeks of age) influenza-infected offspring were significantly smaller in weight (p = 0.002) than uninfected offspring (see Supplemental Material, Table S1), but we saw no effect of arsenic on body weight (p = 0.75).
At day 7 PI (2 weeks of age), offspring infected with influenza had significantly lower body weight (p < 0.001) and higher TGV (p = 0.01) than uninfected offspring (see Supplemental Material, Table S1).
To enrich for transcripts under biotic stress responses, full length-enriched cDNA libraries were prepared from whole plant leaf materials, both uninfected and artificially challenged with pathogen conidiospores.
No inflammation was found in the uninfected animals treated with the plant material.
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