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Pairwise comparisons of each immature stage to the adult stage did not reveal uniformity in expression differences.
This reflects the uniformity in expression stability across the top-ranked genes and is equivalent to using a pairwise variation threshold of 0.23.
The uniformity in expression of top candidate genes suggests that presence of other known mutations (for example, NPM1 and IDH1) may not be a major contributor to expression differences observed between FLT3/ITDH-AR and FLT3/WT samples (Supplementary Table 1).
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The experiments illustrated the pressure to uniformity in groups.
In contrast, some models show uniformity in gene expression from tumor to tumor, including Ras initiated tumors that ordered into a single cluster.
Average expression intensity of the cluster 4 genes was much higher than that of the cluster 1 genes and there was uniformity in the expression of the cluster 4 genes.
The reference gene used was ACT1, which showed excellent uniformity in the expression levels in these fermentation conditions [ 50], and all the reactions were done in a LightCycler® 480 Real-Time PCR System.
The hsp70 promoter (Blechinger et al., 2002) was used to assess the achievable uniformity in reporter gene expression after injection.
To objectively quantify tissue-specificity, we used the concept of entropy (HT) to measure the deviation in expression uniformity for a given transcript across multiple tissues [17].
Mr. Gilbert, showing a good grasp of the whole, laid out the architecture neatly, but a certain uniformity in the pacing and expression made for a long night.
The Gapdh gene was chosen as a reference gene for normalization, since its expression levels are comparable between control and transgenic samples; comparisons to other commonly used reference genes, such a cyclophilin or Polε4 confirmed the uniformity of expression levels in different experimental groups for the Gapdh reference.
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