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Therefore, to obtain more uniform sequencing coverage, we prepared all subsequent libraries from polyA-enriched RNA.
Here, we developed a Bead-based Normalization for Uniform Sequencing (BeNUS) procedure using three steps of bead purification.
Our results indicate a high degree of fractionation in many samples and uniform sequencing coverage across genomes.
Rare variant burdens in cases and controls were not obtained using a uniform sequencing strategy, which could influence estimated prior odds.
Assuming a uniform sequencing process, the number of reads that map to a particular region should be Poisson distributed and proportional to the copy number of the reads.
Overall, the number of transcripts and genes found in three groups are very similar indicating a uniform sequencing depth in the various groups.
Similar(52)
Uniform sequence data among multiple sequenced clones was further proof of the absence of related GLRaVs in the accession of cv.
The noise samples are obtained by transformation functions performed on pseudo-random uniform sequences.
Fig. 7 2D illustration of the superior space-filling properties of a sorted Sobol sequence, compared to a sorted IID uniform sequence.
We employed a symmetric in silico design to obtain a uniform sequence for a stackable repeat while maintaining the desired curvature geometry.
The performance of the NGS technologies at low sequence coverage is correlated with per-base sequence coverage uniformity; where Roche 454, with the most uniform coverage, performs the best and suggests that for all the NGS technologies, achieving more uniform sequence coverage would result in considerably higher performance at lower coverage.
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