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The oriented binding of the target protein to the SAM during formation of the MIP generates uniform binding sites.
R136K CBD showed essentially uniform binding to collagen and its distribution was dependent on that of the collagen scaffold.
This work suggests that this particular system exhibits uniform binding with respect to ground state and transition state and provides insight into the challenge of re-engineering a Mg2+-dependent DNAzyme with enhanced catalytic activity.
In this case we observed uniform binding of EB1-GFP throughout the microtubule lattice, with no preferential binding to seeds, extensions or ends (Figure 2).
In short, the design focuses on uniform binding affinities on all probes and a very high coverage of intergenic regions (down to 7 nt resolution in intergenic regions and 19 probes pr coding region, similar coverage on each strand).
The GC-content is closely related to the Tm score and OligoWiz 2.0 would still select probes with uniform binding affinities but the optimal hybridisation temperature would be different and there are possibilities of a decrease or increase of cross-hybridisation due to the GC-content.
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I didn't know where to begin; I was drawn to complete works in uniform bindings, because I thought they would be a series, like the ones I had loved in the children's section: "Anne of Green Gables" or "The Naughtiest Girl in the School".
The advantages of label-free imaging of chromosomes using STXM includes avoiding of the concerns such as non-uniform binding of labeling agents and photo-bleaching.
After the reign of Constantine, the Roman emperor who effectively made Christianity the religion of the empire, the great ecumenical synods occupied themselves essentially with the task of creating uniform formulations binding upon the entire imperial church.
It assumes a single, reversible adsorption step to uniform cellulose binding sites without interactions among cellulases.
The sprout is situated in a cylindrical domain consisting of an acellular extracellular matrix (ECM) that contains uniform HSPG binding sites for VEGF.
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