Pulse is another way to understand "The King of Limbs," and, by extension, this unfolding phase of Radiohead.
The minor unfolding phase, which appears as a lag during fluorescence-detected unfolding, is consistent with an on-pathway intermediate (I).
For Ala134Val, the initial unfolding phase was too flat to allow determination of the midpoint of the unfolding phase.
Indeed, this mutant showed a relatively high level of fluorescence at low temperatures, i.e. in the 20 30°C range preceding the initial unfolding phase (Figure 3).
The presence of I in eIGPS-Δβ7α7-N231A is verified by the observation of a slow kinetic unfolding phase, whose relaxation times decrease with increasing final denaturant concentration [19], when eIGPS is subjected to an unfolding jump from 0 to 3 M urea where I is expected to be highly populated.
Because the amplitude of the unfolding phase is proportional to the population of N from which the reaction initiates, the decrease in the amplitude as a function of increasing initial urea concentrations (Figure S1) can be fit to a two-state model, N ▒ I, to extract the stability, ΔG°NI, and the urea dependence of the stability, mNI (Dataset S1).
But officials involved in the planning said a firm estimate remained impossible because the sale would unfold in phases and would be likely to change along the way as weapons packages, battlefield-management systems and service contracts were decided.
Although the expression changes between the mouse strains were quite different, they unfolded in phases over time in both strains.
The trial, which started in late February, is unfolding in two phases.
The American Academy of Sleep Medicine describes the experience of sleep as unfolding in four phases, culminating in REM (rapid eye movement) sleep.
