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High pressure (in the kbar range) and alkaline pH were used to disassemble fibrils to collect information on the hierarchic pathway by which distinct β-sheets sequentially unfold using the unique possibility offered by high-pressure Fourier transform infrared spectroscopy.
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Specifically, the spectra unfolding using the MCNP code are applied to determine the neutron flux in the beam tube.
These activation maps were next assigned to 3D surface models of the cortical sheet, and then unfolded, using the Brain à la Carte software package.
And if you want a bit of role play to find out what it may have felt like, you can experience events as they unfolded using the real-time calendar.
After the subtraction of the baseline, data were normalized for protein concentration and fitted according to a non-2-state thermal unfolding model using the software MicroCal addin for OriginLab (Origin Lab Corporation, Northampton, MA).
Buffer baselines were subtracted and data were normalized for protein concentration and fitted with a non-2-state thermal unfolding model using the software supplied by MicroCal (Origin 7).
This database was compiled in real-time as protest events unfolded using reports from the top 100 newspapers in Latin America (see http://www.4imn.com/).
Catalyzed unfolding shares many features that characterize the mechanical unfolding of proteins using the atomic force microscope (AFM).
One work in the current Ailey repertory, Robert Battle's "Unfold," uses Leontyne Price's recording of "Depuis le jour" from Charpentier's "Louise".
Protein unfolding was monitored using the SYPRO orange dye (Invitrogen), which binds to hydrophobic regions on the protein during the unfolding process.
We unfolded the measured spectra by using the multidimensional unfolding method based on Bayesian theory, and the unfolded spectra were compared with current hadronic-interaction models.
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