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Unexpectedly, sequence analysis of some resistant individuals revealed a duplication of the ace-1 gene that was observed in both A. gambiae s.s.s
Unexpectedly, sequence analysis considering the one in frame with the lacZ gene and the adaptor sequence including the EcoRI restriction cloning site (GAATTCGGCACGAG) used to clone the 5'3' cDNA fragments in the λZAP vector revealed a stop codon (TAA in T. solium and E. multilocularis and TGA in the others cestodes) 12 amino acids downstream of the reading frame.
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Unexpectedly, sequencing of the internal spacer sequences of individual repeat elements from multiple isolates showed conservation at the sequence level within deep evolutionary lineages of S. aureus.
Thrombin inhibitor and Evasins were over expressed in the early to mid library, while unexpectedly sequences related to cement (64P) were mostly expressed in the mid library.
Unexpectedly, a sequence and structural analysis of symmetric subdomain modules within an abundant and ancient globular fold, the β-trefoil, reveals that modular evolution is not simply a relic of the ancient past, but is an ongoing and recurring mechanism for regenerating symmetry, having occurred independently in numerous existing β-trefoil proteins.
Unexpectedly, the sequence did not closely match any characterized Babesia species.
During preliminary analysis of the transcriptome assemblies, we observed a few orthologs with unexpectedly high sequence similarity (>99%) among species.
Unexpectedly high sequence conservation was observed in the promoter region of two phloem-expressed SCAmpPs that encode very distinct predicted cyclic products.
This vertical stripe is due to unexpectedly high sequence identity between B. subtilis guanosine reductase (GuaC) and homologous proteins in several strains of the Proteobacterium Helicobacter pylori.
Unexpectedly, the sequence non-specific ARE4 in 3′-UTR (to a smaller extent, in ORF) decreased mRNA half-life more strongly than the consensus-matching AREs.
Nucleotide-resolution GERP scores can then be used to identify runs of unexpectedly constrained sequence, which can also be a useful regional annotation: these runs are defined as 'constrained elements'.
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