Interestingly, the 41 combinatorial profiles were distinctively distributed in the three Cav1.2 libraries and the one named "heart 1 50" contained unexpected splice variants.
When the cDNA clones were sequenced and compared to other plant hexokinases we found several unexpected splice variants (Additional file 8: Table S5).
In conclusion, an unexpected HCV variant, G2j, was found at a relatively high frequency in Venezuela, and could have been introduced into the country with slaves from West Africa.
The abundance of the winter vrn-h3 allele in cultivated spring growth habit germplasm was unexpected since allelic variants at the VRN-H3 locus were previously reported only in exotic barley genotypes [ 17].
However, its advantage is also its main handicap: high resolution allows the detection of a higher percentage of abnormalities than in a conventional karyotype but may also pick up unexpected findings and/or variants that are of unknown clinical significance [ 18].
The unexpected observation that variant PRC1 complexes can nucleate PRC2 to establish a polycomb domain de novo suggests that a feature associated with variant complex occupancy, perhaps H2AK119ub1, is responsible for this activity.
This result was not unexpected as the RAVEN variants comprise known regulatory, rather than disease-causing, variants, and as such would not be expected to be discriminated by the disease variant model.
It remains possible that the association between the variant and unexpected infant death represents a complex interaction of the variant with environmental risk factors including sleep position, bed-sharing, prenatal and postnatal tobacco smoke exposure and food security.
Finally, we describe recent rare variant association findings, highlighting the unexpected conclusion that most rare variants have modest-to-small effect sizes on phenotypic variation.
This was unexpected, since the radioligand variants of the probe QE as well the dye DY-754 showed a renal elimination as well but only a minimal kidney retention [20, 23].
