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CHIAS IV software [ 43] takes advantage of this uneven staining pattern and was used to generate a distinctive CP profile for each of the nine switchgrass chromosomes.
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However, some publications address the possible influence of field selection of TMAs on the observed immunopositivity, especially with regard to uneven staining and expression patterns (Gillett et al, 2000; Wärnberg et al, 2008; Couvelard et al, 2009).
Chromosomes exhibit uneven staining due to variation in condensation pattern (CP) along their length.
However, the complex cellular structure, presence of imaging artifacts, uneven staining variation make the task challenging.
This staining pattern was detected mainly in tumour microvessels exhibiting endothelial proliferation (Figure 2G).
The staining pattern was analyzed in combination with the morphology of the cells.
The staining pattern was identical to the positive control using the 4B7 mAb.
CONCLUSIONS: This study demonstrates a statistically significant difference in CD10 staining pattern between TE and BCC.
However, the staining pattern showed marked contrast under the fluorescein microscope.
Figure 12 shows a typical staining pattern.
Most mutant cells showed relatively light or uneven staining, and disordered structures or fractured organelles were found.
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