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However, uneven sequence coverage, contaminated DNA and chimeric reads bring new assembly challenges.
Here, we review recent developments and the challenges need to be addressed in single-cell metagenomics, including potential contamination, uneven sequence coverage, sequence chimera, genome assembly and annotation.
The results from data-mining sequences in GenBank, notwithstanding the drawbacks of using such data (e.g., unreliable identifications and uneven sequence quality [18]) and the relatively crude nature of the BLAST search engine, indicated that trnH-psbA was successful at returning a correct match.
The heuristic rules we implemented (" Materials and methods") took into consideration uneven sequence coverage.
This leads to differences in amplicon presentation and uneven sequence coverage.
Uneven sequence coverage between insertions and individuals meant that not all insertions could be assembled for each individual.
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Each method used for single-cell genomic DNA amplification has biases to consider and can be affected by poor coverage or uneven sequencing depth, which will result in noisy and inaccurate read counts.
Giving it three out of five stars, Caryn Ganz from Rolling Stone noted an uneven sequencing among the tracks in The Remix.
Jon Hadusek of Consequence of Sound stated, "A few poor production choices and uneven sequencing do slow the album, but it shows flashes of real brilliance.
Firstly, differences may merely be reflective of uneven sequencing of thymic compartments.
Models for generating uneven sequencing coverage and sequencing errors were developed and applied (see Materials and methods).
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