For example, metagenomic sequencing is not bothered by problems such as cell-sorting, chimeric reads and uneven read coverage associated with single-cell genomics.
Uneven read distribution between indexed libraries is a common problem with increasing degree of multiplexing, raising high requirements on concentration determination.
Transcript levels, however, are highly variable, which results in an uneven read coverage distribution and a variable error rate.
Uneven read coverage broadens the peaks in the k-mer abundance spectra, sometimes causing errors in the assignment of sequences to a particular read coverage.
However, uneven read distribution within a gene, caused by experimental bias in sample preparation and sequencing, would greatly affect the estimation of allele-specific patterns.
However, the 5′ end of a transcript is difficult to distinguish accurately from whole transcriptome RNA-seq data due to uneven read coverage and bias in libraries leading to a low frequency of sequence reads from transcript ends [ 23].
This finding is not surprising, because these loci derive from repetitive AT and GC-rich sequences that may be collapsed or represented by uneven read coverage in Illumina sequences [ 35].
All of them are based on the de Bruijn graph and adapted for uneven read coverage.
Secondly, the highly uneven read coverage and an increased proportion of chimeric reads can be caused by the MDA procedure.
However, this method has its own limitations such as false amplification of contaminates, formation of chimeric reads (Lasken and Stockwell, 2007) and production of uneven read coverage.
Most felt that even though there were moments when Steinbeck was at his best, the blending of philosophy, travelogue and biological recording made for an uneven read: Those critics who looked beyond the narrative portion were impressed by Ricketts' catalogue.
