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To overcome problems resulting from uneven expression of reference genes in normal, tumour-adjacent and malignant tissue, we standardised gene expression by referring to total RNA input and by normalisation with the geNorm protocol.
In addition, reporting the uneven expression of 13 HKG in a panel of normal and tumour human tissues, de Kok et al (2005) have shown that the less variable HKG differed according to the tissue and suggested that it will be important, before experiments, to establish HKG variations clearly to select the best one for quantitative real-time PCR (qRT-PCR) standardisation.
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A morphological analysis of the epidermis revealed that the ΔNp63+/Δ mice have an expanded epidermal basal layer with basaloid cells above the basal epithelium and multiple cell layers with uneven expression patterns of K5, K14, K10, K1 and Filaggrin.
Both diffuse strong expression in the whole cytoplasm and uneven expression (as observed for OAT1) in a number of cells in the duct were observed.
The uneven mRNA expression of CYP1A throughout the liver further underlines how important it is to dissect and sample cells from the same part of the liver in future examinations of gene expression in bulk tissue specimens.
There has been "low and uneven construction of rental units.
The redundancy is partial or uneven, depending on the similarities in expression of duplicated genes (Briggs et al. 2006).
No significantly enhanced POMC expression was found in SLs or in uneven-toned skin despite the significantly higher expression of its receptor, MC1R, in SLs (supplementary material Fig. S1E,F; Fig. 1G,H).
11β-HSD1 is widely distributed in the CNS, albeit with an uneven pattern of expression.
Though these reports are extremely uneven and often fragmentary, they do give a sampling of the literary expression of many and diverse parts of the world.
"My work undermines the uneven economics of Western technocolonialism and the performative rhetoric of Islamic fundamentalism," she writes, through a ficto-feminist-figural expression of power.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com