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Both diffuse strong expression in the whole cytoplasm and uneven expression (as observed for OAT1) in a number of cells in the duct were observed.
In particular, isoforms can have very uneven expression levels (e.g. 1 100), which make it very difficult to identify low-expressed isoforms.
To overcome problems resulting from uneven expression of reference genes in normal, tumour-adjacent and malignant tissue, we standardised gene expression by referring to total RNA input and by normalisation with the geNorm protocol.
A morphological analysis of the epidermis revealed that the ΔNp63+/Δ mice have an expanded epidermal basal layer with basaloid cells above the basal epithelium and multiple cell layers with uneven expression patterns of K5, K14, K10, K1 and Filaggrin.
In exposed fish, this gene also shows an uneven expression throughout the liver, with the highest mRNA levels in sections 4 to 7. The GSTs are a family of phase II detoxifying enzymes that protect against injury from a number of endogenous and environmental chemicals.
In addition, reporting the uneven expression of 13 HKG in a panel of normal and tumour human tissues, de Kok et al (2005) have shown that the less variable HKG differed according to the tissue and suggested that it will be important, before experiments, to establish HKG variations clearly to select the best one for quantitative real-time PCR (qRT-PCR) standardisation.
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Due to uneven gene expression, when comparing non-infected and infected placentas, the housekeeping genes TBP, SDHA and YWHAZ were unsuitable for internal controls.
The uneven mRNA expression of CYP1A throughout the liver further underlines how important it is to dissect and sample cells from the same part of the liver in future examinations of gene expression in bulk tissue specimens.
Third, the V-Model can display uneven granularity expressions in one timeline view (P4).
Temporal information, events, and causality (problem-action) relationships are modeled, and the original context is successfully displayed.> The distinctive features of the V-Model are listed in Table 2.> The V-Model can represent problem-action relations, non-explicit temporal information, and uneven granularity expressions.
Nevertheless, the reflection of his face in the window of a shop is frightening: he sees a red nose and bloodshot eyes, lips pursed up tight over stained and uneven teeth, an expression so lugubrious and so pained it resists his efforts to smile.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com