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In this work, we introduce Composite Kinase Mutation Database (CKMD), a single repository and integrated bioinformatics resource that consolidated and unequivocally mapped all currently available information on genetic variations in protein kinase genes with sequence, structural and functional data.
The BAC U39a, covering the region that UMD unequivocally mapped to chr15, clearly indicated that instead this domain maps to chr23.
In this study, we have unequivocally mapped 575 large-insert DNA clones onto zebrafish metaphase chromosomes using multi-color FISH methods.
Of these putative enhancer sequences 39% mapped to a single locus each (single mappers) in the pig genome and 21% were both single mappers and unequivocally mapped only to the pig genome (i.e. with no match to the mouse genome).
One advantage of RNA-Seq compared to microarray is that transcripts can be unequivocally mapped to a single member of a gene family when sequence is similar but not identical.
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Interpreting ChIP-seq requires that there is a reference sequence that has sufficient polymorphism to unequivocally map short sequence reads.
Performing FISH with BAC and cDNA clones on chromosomes of one representative of the New World monkeys, the black-handed spider monkey, we could unequivocally map distinct PKD1-signals distal in 1q26 (Fig. 4a, AG E, which could be defined as the orthologous region to human chromosome 16 [ 32].
We counted both the number of peptides that mapped unequivocally to a single gene (discriminating peptides) and those that mapped two or more different genes (non-discriminating peptides).
For critical nodes supported by the total evidence, character state changes that mapped unequivocally onto all optimal trees were noted; these are listed in Supplementary Table S1.
Proceeding on the assumption that sequence reads were mapped unequivocally, the most distal SD block also has a high probability of being attached to the nuclear membrane.
All sequences such as repeats and segmental duplications that could not be mapped unequivocally in the human and chimpanzee genome, respectively, were excluded from this alignment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com