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The present work was undertaken to elucidate unequivocally direct visualization of ligand-dependent endocytosis and intracellular trafficking of NPRA by utilizing confocal IF analyses.
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More Athenian aggression followed, unequivocally directed against other Greeks: Carystus, at the southeastern end of Euboea, was forced to join the league.
Moreover, the UN Security Council has clearly and unequivocally directed the DRC government to establish a vetting mechanism to prevent those who have committed human rights violations from joining its administrative apparatus and security forces.
Public authority should limit free speech only when it incites violence or unequivocally provokes direct and harmful discrimination against a vulnerable minority.
To unequivocally demonstrate direct regulation, in vivo binding of the targets by AtGRP7 will have to be demonstrated by precipitating AtGRP7-containing mRNP particles from transgenic plants expressing epitope-tagged AtGRP7 and identification of co-precipitated transcripts.
The induction of TNF-α unequivocally required direct cell contact of the monocytes with T cells, which excludes the possibility that the stimuli of cytokine production are soluble mediators released from the fixed T lymphocytes.
These last few years have unequivocally demonstrated the direct relationship between Western, and particularly American, aggression toward Iran and the strengthening of the Iranian government and its image as an apparent voice for the world's oppressed.
For the remaining six BMVs (BMV2 7), the integration sites could not be unequivocally defined by direct comparison of provirus-containing and provirus-free strains, due to unavailability of the genomic sequences for the latter group.
Future studies to delete Ehd3 and Ehd4 in a cell type-specific manner (endothelium or podocytes) using specific Cre-recombinase expressing transgenic mouse lines will help unequivocally distinguish the direct versus indirect effects of endothelial EHD3 and EHD4 loss on the podocyte.
Observation of no or negligible amounts of the sultam 3b or sulfonamide 3c unequivocally confirmed the direct involvement of the protein-coordinated heme and properly folded P450, respectively, for the observed catalytic C H amination activity.
Although not readily detected in wild-type plants, it cannot be unequivocally stated that this direct synthesis pathway of iso-sinapic acid to iso-sinapyl alcohol doesn't exist in such plants at very low flux and metabolite concentrations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com