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The specificity of the probes was demonstrated in hybridizations with RNA samples, in which we were able to unequivocally differentiate miRNAs with only single base differences, for example hsa-miR-133a and hsa-miR-133b, and the human and mouse forms of miR-155 (Fig. 1A).
However, it has been reported that C. dubliniensis, C. parapsilosis, C. tropicalis can also form germ tubes/pseudohyphae in serum and their sugar assimilation patterns are similar to that of C. albicans, hence chances of incorrect diagnosis are high without a test that can unequivocally differentiate between these related species in a short time [4].
Despite these characteristic imaging features, none of these can unequivocally differentiate CNS lymphoma from other brain lesions [ 4].
In conclusion, imaging has very definite limitations and cannot readily or unequivocally differentiate between RS and SL.
Finally, the rbcL and trnL sequences can be used to unequivocally differentiate the populations of the Gelbison massif from those of the Calabrian Apennines in a taxonomic context.
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The approach aims to (i) identify gene sets associated to different pathological states analysed together; (ii) identify a minimum subset within these genes that unequivocally differentiates and classifies the compared disease subtypes; (iii) provide a measurement of the discriminant power of these genes and (iv) identify links between the genes that characterise each of the disease subtypes.
Since epithelial cells have common functions, namely secretion, selective absorption, protection, trans-cellular transport and detection of sensation, finding biomarkers capable of unequivocally differentiating and identifying each of the three cell types commonly found in casework (buccal, vaginal and skin epithelia) is challenging.
In all of these studies, a set of 20-30 miRNAs unequivocally differentiated the PC samples from the normal samples and non-malignant precursor lesions, and specific miRNAs including miR-125b, miR-145, and let-7c were repeatedly detected differentially expressed in different studies.
Restriction fragment length polymorphism (RFLP) generated species-specific patterns of bands visualized in agarose gels, which allowed to differentiate each species unequivocally.
In this paper we report a new molecular method based on single-step multiplex PCR to detect and differentiate simultaneously and unequivocally D. immitis and D. repens on DNA extracted from canine peripheral blood.
In this work, a fast and cost-effective real-time PCR HRMA protocol to detect and differentiate simultaneously and unequivocally D. immitis and D. repens microfilarial DNA extracted from peripheral dog blood samples is described.
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