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Although GST-MVaf1 and GST-MT unequivocally bound EGFP-DLC1 and EGFP-DLC2, Bmf remained in solution.
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In [11] it has been shown that for ensembles of nanoparticles of "core shell" type, under condition of cores to be in a superparamagnetic state, formula (1) practically unequivocally binds the size distribution of Fe3O4 NP in ensemble with a shape of hysteresis curve.
As demonstrated in the online supplementary appendix, this leads unequivocally to the bound on Q used throughout this paper.
In addition, while chromatin immunoprecipitation/micro-array studies using chromosome panels unequivocally revealed γ-H2AX bound to telomeric and subtelomeric sequences [ 5], other non-telomeric genomic sequences may not bind sufficient γ-H2AX to allow detection, leading to underestimation of non-telomeric γ-H2AX.
For our experiments, an anti-myc antibody was bound to the AFM tip to unequivocally detect GLUT4-myc-GFP on the surface of the cells.
Our use of pronuclear assembly in egg extract and fractionation of staged embryos into soluble and chromatin fractions permitted us to unequivocally assign distinct abundances to both undeposited and chromatin bound histones using high resolution mass spectrometry.
The three abundant proteins that bound specifically to DNA, and not to biotinylated lipoprotein pam-3-cys, were unequivocally identified as Ku70, Ku80 and DNA-PKcs.
Toronto bound".
To unequivocally determine if CES can bind to G-box motifs, electrophoretic mobility shift assays (EMSAs) using the CPD-promoter fragment to which CES had bound in ChIPs as a probe (radioactively labelled), recombinant glutathione S-transferase (GST)–CES and cold competitor oligonucleotides were performed.
We next used Western blotting to demonstrate unequivocally that the SMA copolymer extracted integral membrane proteins from the mitochondrial membranes, and did not simply release proteins that were peripherally bound.
Time bound.
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CEO of Professional Science Editing for Scientists @ prosciediting.com