However, the analysis of species-specific plasmidic repeats is unequivocal because plasmid transfer between different bacterial species is a common phenomenon.
In Cyanobacteria, the only unequivocal example of horizontal transfer is in Nodularia spumigena CCY9414, where CrtP has been replaced by a related ortholog, likely without phenotypic divergence.
Similarly, the only unequivocal example of horizontal transfer within the linear xanthophyll-producing Proteobacteria is that of CrtA from the Bacteroidetes, in which it functions as a hydroxylase, into Rubrivivax gelatinosus and Hoeflea phototrophica.
It is not until more relevant humanized xenograft mouse models and phylogenetically related large animal species were used to optimize gene transfer methodologies that unequivocal clinical successes were achieved.
Here we present unequivocal experimental proof that microscale cofactor- and membrane-less, direct electron transfer based enzymatic fuel cells do produce significant amounts of electrical energy in human lachrymal liquid (tears).
It seems, therefore, that establishing an unequivocal relationship between rpoB genotype and RIFR phenotype will require the site-directed transfer of selected RIFR-conferring mutations into sequenced Mtb strains.
Although our analysis yielded other examples of likely gene transfer, the evidence of a high rate of operon loss in multiple clades makes unequivocal distinctions difficult.
Although unequivocal evidence is not (yet) available, expert opinion is clear: critically ill patients should preferably be transferred by a specialized retrieval team.
It now seems unequivocal that mtMutS arose once during octocorallian evolution, in the common ancestor of all extant lineages, through a horizontal gene transfer event from either an epsilonproteobacterium or a nucleocytoplasmic large DNA virus.
Electron micrographs of alveolar tissue, of lining layers of captive bubbles, and scanning force micrographs of surfactant films transferred on mica plates reveal a complex structural pattern which precludes so far the formulation of an unequivocal hypothesis.
