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It is tempting to speculate that the unequivocal cell therapy response may be masked early on by the combined and potentiated effect with the endogenous compensatory mechanisms elicited by the acute myocardial ischemia and one cannot exclude the possibility that the added value of a higher number of cells may be measurable beyond 4 weeks post infarction and the cell treatment.
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The observers agreed that characteristic features of CD include the presence of unequivocal dysplastic cells, similar in appearance to traditional LGD, involving any part, or all of the length, of the crypt in the absence of intercrypt surface epithelial involvement.
However, four patients had unequivocal B-cell disease and unmistakable L1 morphologic type, whereas one and had L3 morphologic features and had non-B-cell disease.
Chromogenic in situ hybridisation signals were counted in a minimum 30 morphologically unequivocal neoplastic cells.
A bone marrow aspirate or trephine biopsy containing unequivocal tumour cells.
Signals were evaluated at 400 × magnification and 630 × magnification, and 30 morphologically unequivocal neoplastic cells in each core were assessed for the presence of the FGFR1 gene signals.
Reasons for non-informative results were: lack of tissue on the TMA, absence of unequivocal tumor cells, or non-interpretable hybridization data.
Signals were evaluated at × 400 and × 630 and 60 morphologically unequivocal neoplastic cells were counted for the presence of the gene probe signals.
Immunostaining was evaluated at least on 10 microscopic fields at × 200 by two independent pathologists and unequivocal immunostained cells were counted.
Therefore, subsequent conversion of these cells into functional endocrine cells provided unequivocal evidence that endocrine cell-free human adult CD133+ ductal cell fraction can be converted into islet endocrine cells.
Given the lack of unequivocal neural stem cell markers in vivo, we tested for stem cell behaviour.
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