To assess performance in quantification of mixed infections, plasmids were mixed to give unequal mixtures for three targets at 200 000, 20 000 and 200 gene copies/reaction.
Thus, I would like to invite the authors to comment on the expected performance of HRM assays when analyzing unequal mixtures.
The capacity to analyze unequal mixtures will depend on the analysis method.
Thus, besides good specificity, sensitivity and the ability to handle (unequal) mixtures are important performance characteristics for RNA assays.
Based on earlier results that showed that the results of a single large trial were unreliable [ 19], we hypothesized that the meta-analyses methods, including HKSJ, would perform less adequately when the meta-analysis is carried out on a mixture of very unequal-sized studies, e.g. one large and several small trials.
You may think that Margaret Thatcher had achieved much in rescuing her country from decades of decline, but apparently not: Britain had become a divided, squalid, appallingly unequal society which badly needed a mixture of socialism, constitutional reform and the admirably long-termist, "stakeholder" capitalism of Germany and Japan.
Many groups exhibited unequal intensities, indicating that the XOS contained a mixture of several molecules.
Mixture of shock and outrage.
The solution is a mixture of both.
So, it was a mixture of reactions.
