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The agglutination assay was interpreted as positive if agglutination occurred in an undiluted specimen.
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Undiluted specimens of nasal lavage fluid were analyzed using Quantikine enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN) for the proinflammatory cytokines IL-8 and IL-1β.
We found that the undiluted saliva specimen was optimal.
All 58 undiluted negative specimens from persons without Cryptococcus spp. infection remained negative by thermal contrast, which demonstrated maintained specificity.
There are several reports that undiluted and untreated specimens have a much greater incidence of inhibition [ 8, 15].
CA specimens were incubated in undiluted methanol at 4°C overnight, washed 3 times and ground in a glass homogenizer, adding ice cold TE buffer (20 mM Tris-HCl, 10 mM EDTA, pH 7.5) [7.5.
In brief, 200 μL of undiluted and 1 10 dilutions of blood or serum specimens were inoculated onto confluent Vero cells in T25 flasks.
Urine samples were collected in sterile specimen containers, and we tested 0.1 mL of undiluted urine for H. capsulatum var capsulatum antigen detection using a polyclonal rabbit anti-histoplasma IgG antibody EIA (Durkin et al. 1997).
Two aliquots of 0.1 mL of the original and concentrated specimens (heat-treated and untreated, 1 10 diluted and undiluted) were each spread on duplicate plates of modified Wadowsky-Yee selective medium (Oxoid Ltd., Basingstoke, Hampshire, UK).
His passion remains undiluted.
Vast, undiluted space.
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