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RA was very similar to SSE in undiluted matrix solution.
Matrix effects were determined by adding stock solution after extraction of blank mock samples to obtain 300 μg L−1 per toxin in undiluted matrix solution as well as in 1 + 9 (v/ v) and 1 + 49 (v/ v) dilutions (diluted with ACN/H2O 1 + 1 (v/ v)).
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The limits of detection relative to undiluted sample matrix were 8.9 nM (0.41 ppb) and 3.54 nM (0.22 ppb) for nitrite and nitrate, respectively.
The matrix constitutes undiluted Matrigel Matrix Growth Factor Reduced (Matrigel GFR; BD Biosciences, San Jose, CA, USA).
Then, some of the NPs were diluted in the Al2O3 matrix and others were undiluted, both followed by annealing at different temperatures of 700°C, 800°C, 900°C, and 1,000°C.
We used undiluted FBS because it is a challenging sample matrix with extremely high protein content (60 80 mg/mL).
When measuring trace molecules in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response.
Undiluted urine samples were spiked with incremental concentrations of nitrite to characterize the matrix effect.
The desalting procedure was performed on 2 ml undiluted sample aliquots using a HiPrep 26/10 desalting column with a Sephadex G-25 column matrix using 50 mM acetate buffer at pH 5.0 and a flow rate of 10 ml/min.
However, for undiluted samples, SSE values between 88 and 144 % (RSD ≤ 5 %, n = 3) were observed due to matrix components which affect the ionisation process of the coeluting metabolites of interest (see ESM Table S12).
Vast, undiluted space.
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