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These cells showed a level of specialization comparable with normal lung and skin even when undifferentiated (Figure 1B).
The observed proliferating nuclei were in most cases undifferentiated (Figure 3D) or appeared to start to degenerate (Figure 3E), but nuclear structures reminiscent of egg and central cell nuclei were also observed (Figure 3F).
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Results indicated that at day 0, wildtype and Dazl−/− null mutant lines have an approximately 50% methylated, 50% unmethylated pattern, as is expected in undifferentiated mESCs (Figure 8, day 0).
At higher magnification, the vasa signal is easily detectable also in undifferentiated oogonia (Figure 6E and F) and differentiating oogonia seemingly entering into the meiosis (Figure 6G–I').
Cracking of the fruiting-bodies revealed the presence of only undifferentiated asci (Figure 9B).
A shRNA down-regulation of Nur 77 was performed in undifferentiated hMSCs (Figure S6).
Indeed, after sorting, we observed an enrichment of type A undifferentiated spermatogonia (Figure 4K N).
One important finding in this work is the differential expression of Bcl-2 between differentiated and undifferentiated hMSCs (Figure 5).
Staining of actin filaments (with fluorescently-conjugated phalloidin) and microtubules (with α-tubulin antibodies) clearly identified cytoskeletal fibres in undifferentiated HUVECs (Figure 1B; n = 4).
We also observed Flk-1 and CD133 were expressed at high level on undifferentiated hESCs (Figure S1), consistent with previous reports [10], [12].
These tissues exhibited distinct nuclear pleomorphisms, nuclear inclusions, and high mitotic indexes, indicative of an undifferentiated sarcoma (Figure 5C, bottom panel).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com