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At harvesting, the numbers of undeveloped ovules, immature and mature achenes were counted.
Because there is only one ovule in each disc floret (Huang et al. 2012), the sum of mature achenes, immature achenes and undeveloped ovules represent the numbers of disc floret per capitulum.
To investigate closer at what stage the undeveloped ovules are affected, both miro1/miro2-2 and miro1 flowers were emasculated, and the siliques were cleared and observed with DIC-microscopy after 48 hours.
Since co-transmission of both miro alleles through the male gametophyte is nearly absent, the observed undeveloped ovules must be due to a combined effect of the miro1/miro2-2 alleles during female gametophyte development.
In this case, where male co-transmission is close to zero, it is therefore reasonable to assume that some of the structures that are observed as undeveloped ovules in miro1/miro2-2 siliques are fertilized ovules that are arrested during or shortly after fertilization.
Similar(55)
When capsules had matured, we harvested them and categorized ovules (seeds) as matured, undeveloped, eaten or unfertilized.
Undeveloped (immature) seeds were larger than the unfertilized ovules but were smaller than mature seeds.
Togo is small and undeveloped.
Seeds are the mature ovules.
But beyond this they are undeveloped.
But this strand is left undeveloped.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com