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Although the standardisation of molecules is of prime importance, this procedure is usually problem-dependent, and we chose not to introduce any particular (possibly undesired) modification on the input molecules.
These patterns can also be altered during certain library construction protocol methods; Seguin-Orlando and colleagues cited the Taq and Phusion polymerase enzymes, which are integral to Illumina sequencing protocols, as a cause of this undesired modification during library preparation.
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Accumulation inside peroxisomes prevents undesired modifications (e.g. proteolytic processing or glycosylation) and is also in particular advantageous when proteins are produced which are toxic or harmful for the host.
In conclusion, the presented studies display the important role of miR transfer by EVs in the development of heart failure and the protection against undesired modifications of the heart muscle.
O-methyltransferases contribute to the production of a number of non-ribosomal antibiotics [ 33, 34] and catalyse the methylation of hydroxyl group(s) on deoxysugar rings to protect the reactive hydroxyl group from undesired modifications and can alter the solubility and pharmacokinetic properties of the resulting molecule [ 35].
Pichia pastoris strains have been re-engineered to overcome this undesired glycan modification [ 5].
Embedding metal nanoparticles (NPs) into metal contacts at metal Si (MS) interface is an alternative method for modification of Schottky barrier height (SBH) and compared with traditional methods that might result in an undesired alteration of the MS interface, offers a tremendous simplification and adaptation in processing steps.
Performing this modification eliminate undesired worsening in the trajectory and provide smoother start and stop of joint motions.
Inefficient or aberrant operation of these processes often results in low levels of gene modification or undesired sequence changes [5].
More precisely, instead of allowing arbitrary modifications in principle and prohibiting certain undesired behavior, the statements explicitly describe what is allowed to change.
We have followed the optimized protocol presented by Baugh et al. [ 5] which has retained the basis of the Eberwine strategy, but where they have introduced some modifications in order to reduce undesired products.
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