A high density of A. tumefaciens increased the transformation frequency; however, undesirable integrations of the T-DNA such as multiple-copy integrations at a single locus, with or without the vector backbone, or integration at multiple loci also increased in A. aculeatus, as previously reported in Blastomyces dermatitidis (Sullivan et al. 2002) and in Suillus bovinus (Hanif et al. 2002).
In the case of S. pyogenes, an undesirable phage integration (more than 100 kb) into one replichore may cause an unstable genome structure, and this unbalanced genome structure might induce chromosomal inversion between highly conserved regions [ 38].
Again, this is undesirable for on-chip integration.
Recognition of instances where studies or measures are not comparable, or integration is undesirable for other reasons, may minimise the time and effort associated with undertaking pooled individual participant data analyses, as well as suggesting other methods of synthesis that are more appropriate, such as coordinated parallel analyses [ 17].
Together, these experiments ruled out the possibility that pp32 reduced cell proliferation due to 'position-effect variegation' resulting from the random integration of a gene into an undesirable region in the genome.
However, in fracture fixation where strong bone/implant integration of intramedullary implants is an undesirable effect, local application of ZOL to stimulate fracture healing, as it has been described before [ 17], may still be an option.
Also, we use programs that were designed for dating single topologies, rather than programs such as BEAST [ 15], because integration over topological uncertainty can have undesirable effects on the specification of priors on times [ 20].
The integral localized approximation reduces computational time in the sense that it eliminates the undesirable and time consuming quadratures with double or triple integration.
The integration design could minimize extracolumn volume, thus excluding undesirable peak broadening and improving separation performance.
On the other hand, φ80- attB integration can be used for simultaneous inactivation of an "undesirable" gene whose expression decreases the performance of a producer strain.
