Total peripheral leukocyte RNA was extracted from 50 AS patients and 161 healthy controls by the TRIzol reagent method (Invitrogen, Carlsbad, CA, USA), then underwent reverse transcription by use of a reverse transcript kit (Takara, Otsu, Shiga, Japan).
The GP focus group transcripts underwent content analysis to identify common themes, which were ranked in order of priority based on the number of times GPs raised the theme.
Of the sample of calls in which transcripts underwent clinical panel review, in most cases members agreed that the NHSDW advice was clinically justifiable and appropriate, although concerns were raised that advice given had led to insufficient or unnecessary actions in a small minority of cases.
Across all SCN inoculated samples, transcript counts underwent normalization and variance estimation using the DESeq R package.
He underwent three trials, the transcripts of which have been destroyed.
A Southern blot analysis demonstrated that the sfar4-1 and sfar4-2 lines contained single T-DNA insertions, and RT-PCR revealed that the homozygous mutants of sfar4-1 and sfar4-2 harbored SFAR4 transcripts that underwent gene silencing.
However, at the cutoff levels used in this study, we did not see any overlap between the transcripts that underwent alternative splicing and genes that showed altered message levels upon RBM47 reintroduction.
It was therefore assumed that the mutant transcripts probably underwent nonsense mediated decay so that, in the final analysis, the deletion represented a loss-of-function mutation with a non-existing integrin protein in the epidermis of the affected animal.
In fact, E2 had no effect on transcription of the majority of genes (85 out of 154 were unaffected at the level of transcripts) that underwent E2-regulated alternative splicing with or without ERα binding sites.
Furthermore, we found that the miR828-cleaved transcripts of three MYBs underwent phased 21-nt siRNA biogenesis production.
