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Of the 71 viral RNA positive cases, 70 underwent sequencing and 66 (94.3%) were genotype 4, the remaining 4 isolates were genotype 1.
PCR products were purified by AMPure kit (Beckman Coulter) and then underwent sequencing on a GS Titanium 70×75 picotiter plate according to the manufacturer's GS-FLX protocol (Roche Applied Science, Indianapolis, IN).
These individuals underwent sequencing of HNF1A and HNF4A.
Representatives of seven genotypes underwent sequencing and phylogenetic analysis.
by PCR, and positive samples underwent sequencing for speciation.
For confirmatory test for Burkholderia cepacia group, isolates were sent to Miroku Medical Laboratory in Nagano, Japan, and underwent sequencing for 16S rRNA.
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Once the original templates were cleaved and removed, the reverse strands underwent sequencing-by-synthesis.
All IIN patients underwent sequence analysis to identify FRMD7 mutations (see Materials and Methods).
Finally, only representative isolates sent to the WHO Collaborating Centre underwent sequence analysis, so further related or unrelated isolates may not have been analyzed.
PABP is encoded by a single gene in yeast and marine algae but during plant evolution the PABP gene family expanded substantially, underwent sequence divergence into three subclasses, and acquired tissue-specificity in gene family member expression.
PCR products from 3 F4 nymphs underwent DNA sequencing, and the resultant sequence was 100% identical to R. rickettsii strain Taiaçu, available in GenBank (accession no. DQ115890).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com