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Sequence analysis using SELECTON revealed that amino acid position 46 in NDUFC2 underwent positive selection during mammalian evolution, thus reflecting functional potential.
Because NDUFC2 underwent positive selection, we assessed the effect of directed mutagenesis of a positively selected amino acid within NDUFC2 on the interaction with its mtDNA-encoded partner ND4.
In contrast, many genes underwent positive selection in this order and the majority of these sequences are clade-specific.
In contrast, many more genes underwent positive selection in Magnaporthales with only 81/1,211 OGs in this category being shared by all three clades.
The evolutionary analyses indicated that the members of ASPP protein family might be present in a common ancestor of Nematostella vectensis and underwent positive selective in the evolutionary history.
Only model Td had both statistical significance (2ΔlnL = 17.12, p<0.01, df = 1) and ω>1 (ω = 1.25), so branch site models were used to search for amino acid sites that underwent positive selection in branch d.
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All the four residues that underwent strong positive selection during the emergence of Mecopterida are located at the bottom of the deep pocket, which is similar to the LBP of RXR.
The nine codons that underwent a confident shift from positive selection in Treated to negative selection in Untreated are in bold.
However, a large number of 5TGM1 MM cells underwent apoptosis with positive staining of annexin V when cocultured with differentiated MC3T3-E1 cells with mineralized nodules (Figure 5A, right).
Nonviable cells, which underwent necrosis, were positive for PI but negative for annexin V-FITC.
On average, infants found to be HIV-1 positive underwent first DNA PCR testing at an older age (median: 162 days, IQR: 63 221) than infants testing negative (median: 82 days, IQR: 48 164) (p = 0.05).
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