At the stages shown in Fig. 2a, cohorts of 29 43 embryos were taken from their respective culture medium, suspended in a low conductivity media (EP), and underwent OET assay (As defined in the Materials and Methods Section).
Of these, for non-SN evaluation, 119 patients underwent OSNA assay evaluation, whereas 64 had single-section histology.
Central nervous system specimens were taken after patients' deaths and underwent immunofluorescent assay and histopathologic examination for rabies antigens; also, specimens were injected intracerebrally into suckling mice in an attempt to isolate the virus.
Each cohort was left in EP medium, at room temperature, for 24 hours (T = 24 hrs)., and thereafter, each was photographed (800× microscopy) and underwent repeat OET assay.
To better understand whether potential adverse effects on the embryos due to EP and OET were reversible, embryos that underwent initial OET assay (T = 0) at the 1-cell, 2-cell, 8-cell and early blastocyst stages, were recovered from the OET device, returned to incubation in KSOM+AA medium, and photographed every 24 hours thereafter.
After completing a questionnaire, the participants were subjected to medical examinations and underwent basic hematological assay for health status evaluation.
All patients admitted to our ICU between 1 April 2011 and 30 June 2013 who developed SS&SSh and underwent endotoxin activity assay (EAA) measurement were retrospectively evaluated.
On day 3, 48 hr after the treatment, cells were harvested to determine the percentage of the cells that underwent apoptosis through TUNEL assay.
Blood samples were also drawn at the time of surgery from three patients in group 2. Samples obtained from groups 1 4 underwent enzyme-linked immunosorbent assay (ELISA) for bevacizumab.
Leukocyte-derived DNA was assayed and underwent QC procedures at the Mayo Clinic Molecular Genome Facility (Rochester, MN).
