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Fibers in all scaffolds underwent alignment in response to strain; however, the rate and extent of alignment that could be achieved varied as a function of cross-linking.
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These sequences were trimmed and then underwent ClustalW alignment in the MegAlign program.
The differentiated cells (dADSCs) underwent self-alignment in a tethered type-1 collagen gel, followed by stabilisation to generate engineered neural tissue (EngNT-dADSC).
Four knees were initially in excessive varus and underwent correction of alignment, as measured by medial proximal tibial angle (MPTA), from 75.5° ± 8.4° (mean ± SD) to 90.2° ± 2.7° (normal range, 85° 90°).
For the five retrogenes that underwent intronization, we examined the alignments of retrocopies and their corresponding parental sequences to assess whether these introns had disturbed the frame of putative translation inherited from the parental genes (Additional file 11).
ASCs aligned and underwent myogenesis, but their fusion rate increased, as did the number of cells fusing into a myotube as a result of their alignment.
However, Cdc5L-knockdown cells bearing chromosome alignment defects continuously arrested at prometaphase and underwent cell death.
Sequencing products were purified and underwent electrophoresis with the 3100 Genetic Analyzer (Applied Biosystems) and aligned by using the multisequence alignment CLUSTALX version 1.8 (13 ).
Recent studies, however, show that there is no difference in clinical function, alignment and survivorship of the components between the knees that underwent computer-navigated surgeries and those that underwent conventional surgeries [9, 16].
One patient in the autograft group persisted with a varus hindfoot and underwent a calcaneus valgus osteotomy with cannulated screw fixation to restore hindfoot alignment.
Twelve patients in group 1 and eight patients in group 2 underwent posterior lumbar interbody fusion (PLIF) or transforaminal lumbar interbody fusion (TLIF) for sagittal and coronal alignment correction.
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