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What is new is the refinement of methods that make it feasible for most laboratories to undertake the protocol successfully.
The proportion of patients on hypertension protocols undertaking the minimum target days texting met the predefined 50% success criterion for AIM01 (1212/14683 83%) and AIM02 (623/1114, 56%) but not AIM03 (32/208, 15%) or AIM10 (81/173, 47%; see online supplementary data file 1).
The funders had no direct role in developing the protocol or undertaking the study.
We have chosen to present the protocol before undertaking the actual analyses.
A detailed step-by-step protocol for undertaking the proposed screening method is available in Additional file 1.
The modified protocol for undertaking the study in Bangladesh was reviewed by project team members prior to obtaining ethical approval to carry out the study, from the Bangladesh Medical Research Council (BMRC) and the ethical review committee of BIRDEM, Bangladesh.
Extraction steps were undertaken following the protocols of the manufacturer, apart from the DNA elution step, where all samples were eluted with 50 μL elution solution.
A further strength of this systematic review is the development of a peer reviewed, registered protocol prior to undertaking the review.
The application of the Navigation Guide is guided by a detailed protocol developed prior to undertaking the review.
A priori protocol was written before undertaking the review and the PRISMA statement used to guide reporting (17).
Preparation for protein identification was undertaken using the protocol described in Gez S. et al. [37] Peptide mass fingerprints of tryptic peptides were generated by MALDI-time of flight (MALDI-TOF) mass spectrometry using a Voyager DE-STR (Applied Biosystems, CA, US).
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