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This could be due to the loss of miRNA during paraffin embedding process or RNA extraction.
Tumour tissues were paraffin embedded, processed and sectioned.
Therefore, incisors from cKO mice were extracted, paraffin embedded and processed for amelogenin immunostaining (Fig. 6).
Formalin fixed, paraffin embedded routinely processed tissue was studied.
Core biopsies were then fixed in formalin, paraffin embedded and processed according to the standard protocol.
The tissue was then paraffin embedded and processed for immunohistological examination.
The specimens were processed for paraffin embedding in paraplast (BDH, Poole, Dorset, UK) as routine procedure.
Brain and spinal cord slices were processed for paraffin embedding.
Cross sections were processed for paraffin embedding and 4-micron sections were stained with hematoxylin and eosin.
And they were processed for paraffin embedding for histological observation.
Lung tissue was fixed in 4% paraformaldehyde overnight, processed for paraffin embedding, and 5 µm paraffin sections prepared for analysis.
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