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GeneSpring GX7.3 software (Agilent Technologies, South Queensferry, UK) was used to undertake expression analysis.
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Therefore, to test this hypothesis, we undertook expression analysis of these metabolic enzymes.
Among these, qRT-PCR is now commonly used in many laboratories to undertake accurate expression profiling of different candidate genes that have been previously identified, either because they are known to be involved in a specific biological process, or else because they have shown an interesting expression profile in global expression analyses (microarrays).
To identify genes associated with trait deterioration in the entomopathogenic nematode Hb, we undertook an expression profiling study using custom Roche NimbleGen expression arrays that screened over 15,220 transcripts.
Thus, on the assumption that some MDS cases with a normal karyotype might also have reduced expression of a gene or genes within the 20q CDR, we undertook an expression analysis of CD34+ cells from 18 MDS cases with a normal karyotype and the eight cases with a 20q aberration (Supplementary file 1A), compared to CD34+ cells from four normal bone marrow samples.
Receiver-operating characteristic (ROC) analysis was undertaken using expression level for each miRNA in lung cancer patients and cancer-free controls [23].
We next examined the above hypothesis by undertaking the expression analysis of molecules involved in ROS-induced DNA damage signaling and growth arrest of cells.
In this well-characterized model, it has been shown that undernourishment of dams during pregnancy predisposes offspring to later adiposity and hyperinsulinaemia when fed a normal diet [21] but only at an age later than we have undertaken gene expression analysis.
To examine the above hypothesis we undertook the expression analysis of apoptotic protein, caspase-8.
It is therefore absolutely necessary to develop a normalisation strategy before undertaking gene expression studies.
To address this issue, we undertook microarray expression profiling from SCN-enriched tissue punches across a 150 lux LD cycle from Bdr and wild-type mice.
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